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Serdar BİLEN, Nizamettin ATAOĞLU, F. Tülay KIZILOĞLU University of Atatürk, Faculty of Agricultural, Department of Soil Science, Erzurum, Turkey Abstract The objective of this study was to determine the effects of inoculation with Rhizobium isolates and magnesium fertilizer on dry matter, NO3-N and total nitrogen contents of beans (Phaseolus vulgaris) of greenhouse conditions. Soil samples taken from 0-20 cm depth were sterilized and put in pots. Six kg da-1 P2O5 TSP (40-46 % P2O5), and 5 kg da-1 K2O K2SO4 (50-53 % K2O) were applied to all pots, and additionally 5 kg da-1 N urea (45 % N) was added to non-inoculated pots. Soils in pots were inoculated by microbial fertilizer (Rhizobium phaseoli F7, F83 isolates) and different doses of magnesium (0, 3, 6, 9, 12 kg da-1 Mg, magnesium sulfate fertilizer) fertilizer on non inoculation, inoculation and nitrogen application. During the growing period, soil moisture content was kept on field capacity. After two months, crops were harvested, dried and analyzed for dry matter, NO3-N, total nitrogen contents of root, shoot and leaves. Result indicated that amount of dry matter, NO3-N and total nitrogen contents of root, shoot and leaves of plants increased with microbial inoculation and increasing doses magnesium application. Introduction Quantity of fixed nitrogen as biological had been changed with soil, variety of seed, climate and efficient of bacteria which was used in inoculation. Rhizobium bacteria have been fixed 4-12 kgda-1 nitrogen (Alexander, 1961). Gök and Martin (1993) reported that inoculation of bacteria increased N2 fixation and plant dry matter in vetch and trifolium. Brocwell et all. (1987) claimed that inoculation with bacteria and applied 5 kgda-1 nitrogen had been increased 15 % yield in bean. Howle et all. (1987), Tellawi et all. (1986) and Sepetoğlu (1994) obtained that inoculation of bacteria in different soybeans increased dry matter, seed yield, weight of nodulation and number, content of nitrogen in plant and seed. Kızıloğlu (1995) found out less inorganic nitrogen compound caused for increase in nodulation. Magnesium is the structural element of chlorophyll molecule. It is also a cofactor in the process of fosforilization in enzyme activity. Result of fertilization Mg+2 increases nodulation and nitrogen fixation. Beegle (1995) reported that calcium and magnesium are essential secondary nutrients required by crops. Legumes in particular have a high demand for calcium and magnesium. High soil potassium can have a negative effect on the uptake of calcium and magnesium by crops. This antagonism does not usually affect the yield of the crop but it can have a negative effect on crop quality (Beegle, 1995). Low magnesium contents in soils affect plant growth and health. Magnesium fertilization may favorably influence the yield and mineral contents of plants. When levels of this nutrient in soils are low, the addition of Mg to a fertilizer program will frequently improve crop yields (Durlach, 1993). Material and Methods In this research was used three-day-old pure Rhizobium bacteria culture (F7, F15). These bacteria were incubated at 28 oC in YMA (Yeast Extrakt Mannitol Agar) solid medium and determined as Gram (-) by using Gram Dying Method. (Brown et al., 1964; Haktanır, 1986). Culture suspensions were diluted as 1x109 cells in each milliliter by using Dilution Method (Gürgün and Halkman, 1988). Sugar bean seeds were exposed with 5 % H2O2 solution. Three of these sterilized seeds were put in pots. After sprouting, only one plant for each pot permitted to leave and the others were taken out (Kızıloğlu ve Bilen, 1997). Moisture level of soil was measured by mercury tansiometter. After two months of experimental period, crops were harvested.. The amount of nitrate and total nitrogen in the ground root, shoot and leaves parts is measured by Kjeldahl Method (Carter, 1993) and total amount of this N is used as criteria of N2 fixing capacity of bean plants. Analysis of variance (ANOVA) was performed and grouped by LSD procedure. Results and Discussion The results of the variance analysis belonging to the amount of the dry matter, nitrate and total nitrogen of Sugar bean plants which Rhizobium phaseoli inoculation and different doses of the magnesium applied are shown in Table 1. Inoculation of Rhizobium phaseoli isolates and different doses of magnesium fertilization increased dry matter, nitrate and total nitrogen of Sugar bean plants. Inoculated with Rhizobium phaseoli isolates and fertilization increasing magnesium doses of Sugar bean plant were better than not inoculated in terms of dry matter, nitrate and total nitrogen contents. According to increasing magnesium doses had been seen increasing in dry matter, nitrate and total nitrogen in non inoculated, inoculated and nitrogen applications of Sugar bean plants. F83 x Mg4 combinations had the best effect on root, shoot and leaves regarding with dry matter, nitrate and total nitrogen content. As dose of magnesium doses increased, dry matter, nitrate and total nitrogen contents of the plant in root, shoot and leaves increased. Between average dry matter, nitrate and total nitrogen contents of the root, shoot and leaves were more significant (p<0.01). Similar results were also reported that inoculation Rhizobium isolates of some leguminacea were obtained increasing in the number of nodulation, weight of nodulation and nitrogen content of plant and seed (Brocwell et all., 1985; Sepetoğlu, 1994; Tellawi et all., 1986; Howle et all., 1987). High level magnesium in soil increased nodulation and nitrogen fixation of plant (Kızıloğlu, 1995). |